Studying the Effect of Gold Nanoparticles Prepared by Pulse Laser Ablation in a Liquid on Bacterial Activity


  • Noora H. Al-Zobiadi Department of Physics, College of Science, University of Tikrit, Iraq
  • Ammar A. Habeeb Department of Physics Science, University of Diyala, Iraq
  • Awatif S. Jasim Department of Physics, College of Science, University of Tikrit, Iraq



pulsed laser ablation technique, AuNPs, (UV-Vis) spectroscopy, SEM, TEM


 Gold nanoparticles (AuNPs) were synthesized using the pulsed laser ablation technique in liquid. An (Nd: YAG) laser operating at a fixed energy of 600 mJ and a frequency of 4.3 Hz was employed. The absorption spectrum of the synthesized particles was measured using an ultraviolet-visible spectrometer (uv-vis), with peak values observed in the range of (523–530) nm. Scanning electron microscope (SEM) analysis results revealed that the particles exhibited a spherical shape and had an average size ranging from (38 - 92) nm. X-ray diffraction  confirmed the crystalline structure of the material, which was determined to be cubic. Transmission electron microscopy (TEM) images demonstrated that the particle diameter was (31 and 21) nm for the respective frequencies, which corresponded to 1200 pulses. Furthermore, the biological activity of the AuNPs was assessed against positive and negative bacteria.



Nakamura, S., Sato, M., Sato, Y., Ando, N., Takayama, T., Fujita, M., & Ishihara, M. Synthesis and application of silver nanoparticles (Ag NPs) for the prevention of infection in healthcare workers. International journal of molecular sciences, , 20.15: 3620. (2019). https:// doi:10.3390/ijms20153620

Sharma, M., Easha, P., Tapasvi, G., & Reetika, R. SHARMA, Manu, et al. Nanomaterials in biomedical diagnosis. In: Nanomaterials in Diagnostic Tools and Devices. Elsevier, p. 57-83. (2020)‏.

Singh, A., Dubey, S., & Dubey, H. K. Nanotechnology: The future engineering. Nanotechnology, 6.2: 230-3.‏(2019). :

‏ Hosu O, Cernat A and Feier B. Recent approaches to the synthesis of smart nanomaterials for nanodevices in disease diagnosis. In: Nanomaterials in diagnostic tools and devices. Elsevier, p. 1-55.‏ 2020)).

Sing, S. L., Huang, S., Goh, G. D., Goh, G. L., Tey, C. F., Tan, J. H. K., & Yeong, W. Y.. Emerging metallic systems for additive manufacturing: in-situ alloying and multi-metal processing in laser powder bed fusion. Prog. Mater. Sci. 119, 100795 (2021).

Du, H., Castaing, V., Guo, D., & Viana, B. Rare-earths doped-nanoparticles prepared by pulsed laser ablation in liquids. Ceram. Int. 46, 26299–26308 (2020).

Forsythe, R. C., Cox, C. P., Wilsey, M. K., & Muller, A. M.. Pulsed laser in liquids made nanomaterials for catalysis. Chem. Rev. 121, 7568–7637 (2021).

Zhang, J. M., Chaker, M. & Ma, D. L. Pulsed laser ablation based synthesis of colloidal metal nanoparticles for catalytic applications. J. Colloid Interface Sci. 489, 138–149 (2017).

Theerthagiri, J., Karuppasamy, K., Lee, S. J., Shwetharani, R., Kim, H. S., Pasha, S. K., ... & Choi, M. Y. Fundamentals and comprehensive insights on pulsed laser synthesis of advanced materials for diverse photo-and electrocatalytic applications. Light: Science & Applications, 11.1: 250. ‏ (2022).

Kalimuthu, K., Cha, B.S., Kim, S., Park, K.S.J.M.J., Eco-friendly synthesis and biomedical applications of gold nanoparticles: a review. 152:104296.( 2020).

Rahman, A., Rahman, A., Ghann, W., Kang, H. G., & Uddin, J. Terahertz multispectral imaging for the analysis of gold nanoparticles’ size and the number of unit cells in comparison with other techniques. Int. j. biosens. bioelectron, 4: 159-164. (2018). DOI: 10.15406/ijbsbe.2018.04.00118.

Lee, Y.J., Ahn, E.-Y., Park, Y.J. Shape-dependent cytotoxicity and cellular uptake of gold nanoparticles synthesized using green tea extract. Nanoscale research letters, 14: 1-14.(2019).

Borzenkov, M., Chirico, G., Collini, M., & Pallavicini, P Gold nanoparticles for tissue engineering. Environmental Nanotechnology: Volume 1, 343-390 .(2018).

J. Meena, A. Gupta, R. Ahuja, A.K. Panda, S. Bhaskar norganic particles for delivering natural products. Sustainable Agriculture Reviews 44: Pharmaceutical Technology for Natural Products Delivery Vol. 2 Impact of Nanotechnology, 205-241. (2020).

Kalimuthu, K., Cha, B.S., Kim, S., Park, K.S.J.M.JEco-friendly synthesis and biomedical applications of gold nanoparticles: A review. Microchemical Journal, 152: 104296.(2020).

Mirghassemzadeh, N., Ghamkhari, M. and Dorranian, D. Dependence of laser ablation produced gold nanoparticles characteristics on the fluence of laser pulse. Soft Nanoscience Letters, 2013, (2013). DOI:10.4236/snl.2013.34018

Tessaro, L., Aquino, A., Panzenhagen, P., Ochioni, A. C., Mutz, Y. S., Raymundo-Pereira, P. A., ... & Conte-Junior, C. A. Development and application of an SPR nanobiosensor based on AuNPs for the detection of SARS-CoV-2 on food surfaces. Biosensors, 12.12: 1101.(2022).

Rashed, H. H., & Moatasemballah, J. Synthesis and characterization of Au: CuO nanocomposite by laser soldering on porous silicon for photodetector. Al-Nahrain Journal of Science, 20.2: 49-59.(2017). DOI: 10.22401/JUNS.20.2.07.

‏Mansoureh, G., & Parisa, V. Synthesis of metal nanoparticles using laser ablation technique. In: Emerging applications of nanoparticles and architecture nanostructures. Elsevier, p. 575-596. (2018). DOI: 10.1016/B978-0-323-51254-1.00019-1

Mobed, A., Hasanzadeh, M., & Seidi, F. Anti-bacterial activity of gold nanocomposites as a new nanomaterial weapon to combat photogenic agents: Recent advances and challenges. RSC advances,11.55: 34688-34698.(2021). DOI: 10.1039/D1RA06030A.




How to Cite

H. Al-Zobiadi, N., A. Habeeb , A., & S. Jasim, A. (2023). Studying the Effect of Gold Nanoparticles Prepared by Pulse Laser Ablation in a Liquid on Bacterial Activity. Al-Iraqia Journal for Scientific Engineering Research, 2(4), 63–70.